Affinity - Biology-Online Dictionary | Biology-Online Dictionary
Why does a lower Michaelis-Menten constant indicate a higher affinity of the enzyme for the What is the relation between Km and affinity of an enzyme?. affinity definition: The definition of affinity is a strong connection or relationship between people or things. (noun) A married couple is an example of affinity. Affinity marketing is a concept that consists of a partnership between a company ( supplier) and Second, affinity is based on relationships creating a sense of recognition in Promoting prescription discount cards and medical negotiation services to an affinity group that was created to promote lower healthcare costs is an.
For instance, very enthusiastic baseball fans support their team for a deserving cause, identify themselves in their team, and personally benefit from discounts and advantages.
The affinity group's leader generally send a personal written communication to the affinity group membership to strengthen credibility and the members' confidence in the product.
Other marketing such as newsletters and electronic social media marketing are also used. Promoting prescription discount cards and medical negotiation services to an affinity group that was created to promote lower healthcare costs is an example.
Similar to the above example, a package of medical service savings coupons and discounts as well as prescription discount cards and coupons sold to the members that are known to struggle with the high cost of insurance and healthcare would be considered and enhancement package.
This may differ from the above, shared incentive concept, in that this medical savings package is valuable to members of affinity groups regardless of the stated purpose of the affinity group. The example of prescription discount cards used by football teams' fans help to figure out affinity marketing's characteristics.
The first feature as it is stated above is the third party endorsement. In this case, the fact of endorsement by using the club's logo is just considered an endorsement and is passively marketed through newsletters and possibly sponsorship signage and mentions as a means to advertise.
It is only when the affinity group is actively involved in marketing the product that this becomes an example of the second affinity marketing features anan shared incentive type of marketing.
The team's manager or captain sends a personalized letter to his fans spurring them to use the prescription discount card. Possibly electronic direct marketing and telephonic marketing can be used to increase utilization of the card. An enzyme with a low Km relative to the physiological concentration of substrate, as shown above, is normally saturated with substrate, and will act at a more or less constant rate, regardless of variations in the concentration of substrate within the physiological range.
An enzyme with a high Km relative to the physiological concentration of substrate, as shown above, is not normally saturated with substrate, and its activity will vary as the concentration of substrate varies, so that the rate of formation of product will depend on the availability of substrate.
Affinity marketing - Wikipedia
If two enzymes, in different pathways, compete for the same substrate, then knowing the values of Km and Vmax for both enzymes permits prediction of the metabolic fate of the substrate and the relative amount that will flow through each pathway under various conditions.
In order to determine the amount of an enzyme present in a sample of tissue, it is obviously essential to ensure that the limiting factor is the activity of the enzyme itself, and not the amount of substrate available.
This means that the concentration of substrate must be high enough to ensure that the enzyme is acting at Vmax.
In practice, it is usual to use a concentration of substrate about 10 - fold higher than the Km in order to determine the activity of an enzyme in a sample.
If an enzyme is to be used to determine the concentration of substrate in a sample e.
The relationship is defined by the Michaelis-Menten equation: A number of ways of re-arranging the Michaelis-Menten equation have been devised to obtain linear relationships which permit more precise fitting to the experimental points, and estimation of the values of Km and Vmax.
There are advantages and disadvantages associated with all three main methods of linearising the data.Oxygen Hemoglobin Dissociation Curve Explained Clearly (Oxyhemoglobin Curve)
The Lineweaver-Burk double reciprocal plot rearranges the Michaelis-Menten equation as: These are the points at which the precision of determining the rate of reaction is lowest, because the smallest amount of product has been formed.
The Eadie-Hofstee plot rearranges the Michaelis-Menten equation as: However, it has the disadvantage that v, which is a dependent variable, is used on both axes, and hence errors in measuring the rate of reaction are multiplied, resulting in lower precision of the estimates of Km and Vmax The Hanes plot rearranges the Michaelis-Menten equation as: